Biological Responses Of Fibroblasts And Osteoblasts On Modified Bioactive Titanium Implant Surfaces

Background: Calcium-phosphate bioactive coatings have been suggested to improve the biological response of titanium. We have developed a new manufacturing protocol that enables integration of these bioactive materials in a titanium scaffold, overcoming the mechanical limitations of coatings. However, the biological behavior of these new materials is not yet described. Objectives: The aim of this study was to evaluate the behavior of human fetal osteoblasts and human gingival fibroblasts in contact with Titanium (Ti), Ti with 5% hydroxyapatite (TiHA) or Ti with 5% beta tricalcium phosphate (Ti-bTCP). Materials and methods: Ti, TiHA and Ti-bTCP discs were produced with homogeneous surface roughness. Human fetal osteoblasts and human gingival fibroblasts were cultured on discs for 14 days using previously established methods. Morphology and cellular adhesion were analyzed using scanning electron microscopy (SEM) after 1 and 7 days. Cell viability and proliferation were evaluated at pre-defined time-points (1,3,7 and 14 days) using a commercial resazurin-based method. Alkaline phosphatase (ALP) activity was evaluated at 7 and 14 days for osteoblasts. All results were presented as mean fluorescence intensity (AU) u00b1 standard deviation (SD). Mineralization patterns were evaluated using hydroxyapatite fluorescence staining. Group comparisons were tested using Anova (Tukeyu2019s post-hoc) using appropriate statistical software and significance was set p